Staff profile

Research Interests

Chemical physics and biophysics. I am interested in the application of optical spectroscopy and microscopy for investigations of biological systems and organic materials for display applications, molecular electronics and sensing of the biological environment. The work is interdisciplinary and relies on collaborations with colleagues active in synthetic chemistry and cell biology.

Modulation of the excited state by the di-electric medium

We are interested in how the medium affects the excited state decay and this is monitored using mainly time-resolved emission techniques. The aim of this work is to explore the sensing ability in organic chromophores and organo metallic complexes. 

Luminescence imaging and time-resolved emission microscopy

We are using luminescence imaging to study bio-active materials using emissive probes. The excited state decay of organic fluorescence probes and organo metallic complexes is monitored using time-resolved luminescence microscopy.

Time-gated detection is illustrated above. In the experiment the short lived fluorescence from the matrix has decayed before the gate is turned on and the photoluminescence from the metal centered emission is exclusively monitored. In combination we are also using conventional photon counting techniques to study the organic fluorescence. We are using microscope detection enabling single cells to be studied. We can study the excited state decay of many different types of emissive probes over a very wide dynamic range over 100 orders of magnitude.

Polarised luminescence spectroscopy for molecular recognition and sensing.

We are pursuing circularity polarised luminescence spectroscopy as a tool to study biological systems. This methodology can be used to investigate stereo specific inter-molecular interactions between functionalised optical probes and chiral biological macromolecules such as proteins.

 

References

• Anil Suri, Rui Campos, Darius G. Rackus, Nicholas J.S. Spiller, Christine Richardson, Lars-Olof Pålsson and Ritu Kataky (2011). Liposome-doped hydrogel for implantable tissue. Soft Matter. 7, 7071-7077.
• Lisa Murphy,Aileen Congreve,Lars-Olof Pålssonand J. A. Gareth Williams (2010). The time domain in co-stained cell imaging: time-resolved emission imaging microscopy using a protonatable luminescent iridium complex. Chem. Comm. 43, 8743 - 8745.
• Lars-Olof Pålsson, Benjamin S. Nehls, Frank Galbrecht, Benjamin A. Coombs, Fernando B. Dias, Tony Farrell, Ullrich Scherf, and Andrew P. Monkman (2010). Excited state dynamics and optical characteristics of oligophenyl based cruci forms in solution and solid state. J. of Phys. Chem. B, 114(40), 12765 - 12776.
• Lars-Olof Pålsson, Changsheng Wang, Andrei S. Batsanov, Simon M. King, Andrew Beeby, Andrew P. Monkman and Martin R. Bryce (2009). Intramolecular charge transfer in molecular wires comprised Oligoyne-Bridged Oxadiazol-Triphenylamine Dipoles: Synthesis, x-Ray Crystal Structure, Solution Electrochemistry and opto-electronic properties. Chem. Eur. J. 16, 1470-1479.
• Evan G. Moore, Géza Szigethy, Jide Xu, Lars-Olof Pålsson, Andrew Beeby and Kenneth N. Raymond (2008). 3,2-HOPO Complexes of Near Infra-Red (NIR) Emitting Lanthanides: Sensitization of Ho(III) and Pr(III) in Aqueous Solution. Angewandte Chemie Interdit. 47, 9500-9503.
• Lars-Olof Pålsson, Robert Pal, Benjamin S. Murray, David Parker and Andrew Beeby (2007) Two-photon absorption and photoluminescence of europium based emissive probes for bioactive systems. J. of Dalton Trans. 2007, 5726-5734.

• Chemical Physics
• Biophysics
• Optical Spectroscopy

Chapter in book

• Palsson, L., Spangfort, M., Gulbinas, V., & Gillbro, T. (1993). Chlorophyll b to chlorophyll a energy transfer in the isolated light-harvesting complex II, LHC II, of green plants. In J. E. Korppi-Tommola (Ed.), Laser Spectroscopy of Biomolecules: 4th International Conference on Laser Applications in Life Sciences (107-110). SPIE
• Sharkov, A., Khoroshilov, E., Kryukov, I., Palsson, L., Kryukov, P., Fischer, R., …Gillbro, T. (1993). Femtosecond excitation transfer processes in biliprotein trimers. In J. E. Korppi-Tommola (Ed.), Laser Spectroscopy of Biomolecules: 4th International Conference on Laser Applications in Life Sciences (136-145)

Journal Article

• Szablewski, M., Thompson, R. L., & Pålsson, L. (2022). Modulated Fluorescence in LB Films Based on DADQs—A Potential Sensing Surface?. Molecules, 27(12), Article 3893. https://doi.org/10.3390/molecules27123893
• Guillen-Garcia, A., Gibson, S., Jordan, C., Ramaswamy, V., Linthwaite, V., Bromley, E., …Cann, M. (2022). Allophycocyanin A is a carbon dioxide receptor in the cyanobacterial phycobilisome. Nature Communications, 13, Article 5289. https://doi.org/10.1038/s41467-022-32925-6
• Smith, P. O., Black, D. J., Pal, R., Avó, J., Dias, F. B., Linthwaite, V. L., …Pålsson, L. (2021). Applying TADF Emitters in Bioimaging and Sensing—A Novel Approach Using Liposomes for Encapsulation and Cellular Uptake. Frontiers in Chemistry, 9, Article 743928. https://doi.org/10.3389/fchem.2021.743928
• Sharples, G. J., Heddle, J. G., Kozak, M., Taube, M., Hughes, T. R., Pålsson, L., …Chakraborti, S. (2020). A bacteriophage mimic of the bacterial nucleoid-associated protein Fis. Biochemical Journal, 477(7), 1345-1362. https://doi.org/10.1042/bcj20200146
• Crucho, C. I., Avó, J., Diniz, A. M., Pinto, S. N., Barbosa, J., Smith, P. O., …Dias, F. B. (2020). TADF Dye-Loaded Nanoparticles for Fluorescence Live-Cell Imaging. Frontiers in Chemistry, 8, Article 404. https://doi.org/10.3389/fchem.2020.00404
• Jõgela, J., Uri, A., Pålsson, L., & Enkvist, E. (2019). Almost complete radiationless energy transfer from excited triplet state of a dim phosphor to a covalently linked adjacent fluorescent dye in purely organic tandem luminophores doped into PVA matrix. Journal of Materials Chemistry C Materials for optical and electronic devices, 7(22), 6571-6577. https://doi.org/10.1039/c8tc05325a
• Pal, R., Barker, A. C., Hummel, D., & Pålsson, L. (2019). In Vitro and in Cellulo Sensing of Transition Metals Using Time-Resolved Fluorescence Spectroscopy and Microscopy. Journal of Fluorescence, 29(1), 255-263. https://doi.org/10.1007/s10895-018-2335-z
• Townsend, P., Dixon, C., Slootweg, E., Sukarta, O., Yang, A., Hughes, T., …Cann, M. (2018). The intracellular immune receptor Rx1 regulates the DNA-binding activity of a Golden2-like transcription factor. Journal of Biological Chemistry, 293(9), 3218-3233. https://doi.org/10.1074/jbc.ra117.000485
• Foster, A., Pernil, R., Patterson, C., Scott, A., Pålsson, L., Pal, R., …Robinson, N. (2017). A tight tunable range for Ni(II) sensing and buffering in cells. Nature Chemical Biology, 13(4), 409-414. https://doi.org/10.1038/nchembio.2310
• Sansee, A., Meksawangwong, S., Chainok, K., Franz, K., Gal, M., Palsson, L. -., …Kielar, F. (2016). Novel aminoalkyl tris-cyclometalated iridium complexes as cellular stains. Dalton Transactions, 45(43), 17420-17430. https://doi.org/10.1039/c6dt02776h
• Fenyk, S., Dixon, C., Kittens, W., Townsend, P., Sharpies, G., Pålsson, L., …Cann, M. (2016). The tomato Nucleotide-Binding Leucine-Rich Repeat (NLR) Immune Receptor I-2 couples DNA-Binding to Nucleotide-Binding Domain Nucleotide Exchange. Journal of Biological Chemistry, 291(3), 1137-1147. https://doi.org/10.1074/jbc.m115.698589
• Fenyk, S., Townsend, P. D., Dixon, C. H., Spies, G. B., de San Eustaquio Campillo, A., Slootweg, E. J., …Cann, M. J. (2015). The Potato Nucleotide-Binding Leucine-Rich Repeat (NLR) Immune Receptor Rx1 is a Pathogen Dependent DNA-Deforming Protein. Journal of Biological Chemistry, 290(41), 24945-24960. https://doi.org/10.1074/jbc.m115.672121
• Neil, E., Fox, M., Pal, R., Pålsson, L., O'Sullivan, B., & Parker, D. (2015). Chiral probe development for circularly polarised luminescence: comparative study of structural factors determining the degree of induced CPL with four heptacoordinate europium(III) complexes. Dalton Transactions, 44(33), 14937-14951. https://doi.org/10.1039/c5dt02358k
• Bayazit, M., Pålsson, L., & Coleman, K. (2015). Sensing properties of light-emitting single walled carbon nanotubes prepared via click chemistry of ylides bound to the nanotube surface. RSC Advances, 5(46), 36865-36873. https://doi.org/10.1039/c5ra04330a
• Linton, K., Fox, M., Pålsson, L., & Bryce, M. (2015). Oligo(p-phenyleneethynylene) (OPE) Molecular Wires: Synthesis and Length Dependence of Photoinduced Charge Transfer in OPEs with Triarylamine and Diaryloxadiazole End Groups. Chemistry - A European Journal, 21(10), 3997-4007. https://doi.org/10.1002/chem.201406080
• Szablewski, M., Fox, M., Dias, F., Namih, H., Snedden, E., King, S., …Pålsson, L. (2014). Ultrafast Dynamics and Computational Studies on Diaminodicyanoquinodimethanes (DADQs). Journal of Physical Chemistry B (Soft Condensed Matter and Biophysical Chemistry), 118(24), 6815-6828. https://doi.org/10.1021/jp411358d
• Carr, R., Puckrin, R., McMahon, B. K., Pal, R., Parker, D., & Pålsson, L. (2014). Induced circularly polarized luminescence arising from anion or protein binding to racemic emissive lanthanide complexes. Methods and Applications in Fluorescence, 2(2), Article 024007. https://doi.org/10.1088/2050-6120/2/2/024007
• Steffen, A., Ward, R., Tay, M., Edkins, R., Seeler, F., van Leeuwen, M., …Marder, T. (2014). Regiospecific Formation and Unusual Optical Properties of 2,5-Bis(arylethynyl)rhodacyclopentadienes: A New Class of Luminescent Organometallics. Chemistry - A European Journal, 20(3), 3652-3666. https://doi.org/10.1002/chem.201304068
• Kasari, M., Ligi, K., Gareth Williams, J., Vaasa, A., Enkvist, E., Viht, K., …Uri, A. (2013). Responsive microsecond-lifetime photoluminescent probes for analysis of protein kinases and their inhibitors. BBA - Proteins and Proteomics, 1834(7), 1330-1335. https://doi.org/10.1016/j.bbapap.2013.02.039
• Linton, K., Fisher, A., Pearson, C., Fox, M., Pålsson, L., Bryce, M., & Petty, M. (2012). Colour tuning of blue electroluminescence using bipolar carbazole-oxadiazole molecules in single-active-layer organic light emitting devices (OLEDs). Journal of materials chemistry, 22(23), 11816-11825. https://doi.org/10.1039/c2jm31825c
• Crawford, A., Dwyer, A., Liu, Z., Steffen, A., Beeby, A., Pålsson, L., …Marder, T. (2011). Experimental and Theoretical Studies of the Photophysical Properties of 2- and 2,7-Functionalized Pyrene Derivatives. Journal of the American Chemical Society, 133(34), 13349-13362. https://doi.org/10.1021/ja2006862
• Suri, A., Campos, R., Rackus, D., Spiller, N., Richardson, C., Pǻlsson, L., & Kataky, R. (2011). Liposome-doped hydrogel for implantable tissue. Soft Matter, 7(15), 7071-7077. https://doi.org/10.1039/c1sm05530e
• Palsson, L., Nehls, B., Galbrecht, F., Coombs, B., Dias, F., Farrell, T., …Monkman, A. (2010). Synthesis, Excited State Dynamics, and Optical Characteristics of Oligophenyl-Based Swivel Cruciforms in Solution and Solid State. Journal of Physical Chemistry B (Soft Condensed Matter and Biophysical Chemistry), 114(40), 12765-12776. https://doi.org/10.1021/jp1028883
• Murphy, L., Congreve, A., Pålsson, L., & Williams, J. (2010). The time domain in co-stained cell imaging: time-resolved emission imaging microscopy using a protonatable luminescent iridium complex. Chemical Communications, 46(46), 8743-8745. https://doi.org/10.1039/c0cc03705b
• Pålsson, L., Wang, C., Batsanov, A., King, S., Beeby, A., Monkman, A., & Bryce, M. (2009). Efficient Intramolecular Charge Transfer in Oligoyne-Linked Donor–π–Acceptor Molecules. Chemistry - A European Journal, 16(5), 1470-1479. https://doi.org/10.1002/chem.200902099
• Palsson, L., Pal, R., Murray, B., Parker, D., & Beeby, A. (2007). Two-photon absorption and photoluminescence of europium based emissive probes for bioactive systems. Dalton Transactions, 5726-5734. https://doi.org/10.1039/b710717j
• Dias, F., Pollock, S., Hedley, G., Palsson, L., Monkman, A., Perepichka, I., …Bryce, M. (2006). Intramolecular charge transfer assisted by conformational changes in the excited state of fluorene-dibenzothiophene-S,S-dioxide co-oligomers. Journal of Physical Chemistry B (Soft Condensed Matter and Biophysical Chemistry), 110(39), 19329-19339. https://doi.org/10.1021/jp0643653
• Palsson, L., Vaughan, H., & Monkman, A. (2006). Polarized optical spectroscopy applied to investigate two poly(phenylene-vinylene) polymers with different side chain structures. The Journal of Chemical Physics, 125, 164701-164707. https://doi.org/10.1063/1.2354473
• Wang, C., Pålsson, L., Batsanov, A., & Bryce, M. (2006). Molecular wires comprising pi-extended ethynyl- and butadiynyl-2,5-diphenyl-1,3,4 oxidiazole derivatives: synthesis, redox, structural and optoelectronic properties. Journal of the American Chemical Society, 128(11), 3789-3799. https://doi.org/10.1021/ja0577600
• Porrès, L., Holland, A., -O. Pålsson, L., Monkman, A., Kemp, C., & Beeby, A. (2006). Absolute measurements of photoluminescence quantum yields of solutions using an integrating sphere. Journal of Fluorescence, 16(2), 267-273. https://doi.org/10.1007/s10895-005-0054-8
• Szablewski, M., Bloor, D., Kagawa, Y., Mosurkal, R., Cole, J., Clark, S., …Palsson, L. (2006). Matrix dependence of blue light emission from a novel NH2-functionalized dicyanoquinodimethane derivative. Journal of Physical Organic Chemistry, 19(3), 206-213. https://doi.org/10.1002/poc.1020
• Palsson, L., Vaughan, H., Smith, A., Szablewski, M., Cross, G., Roberts, T., …Bloor, D. (2006). Guest-host interactions between dichroic dyes and anisotropic hosts. Journal of Luminescence, 117(1), 113-122. https://doi.org/10.1016/j.jlumin.2005.03.017
• Perepichka, I., Perepichka, I., Bryce, M., & Palsson, L. (2005). Dibenzothiophene-S,S-dioxide-fluorene co-oligomers. Stable, highly-efficient blue emitters with improved electron affinity. Chemical Communications, 3397-3399
• Knaapila, M., Kisko, K., Lyons, B., Stepanyan, R., Foreman, J., Seeck, O., …Monkman, A. (2004). Influence Of Molecular Weight On Self-organization, Uniaxial Alignment, And Surface Morphology Of Hairy-rodlike Polyfluorene In Thin Films. Journal of Physical Chemistry B (Soft Condensed Matter and Biophysical Chemistry), 108(30), 10711-10720. https://doi.org/10.1021/jp049067p
• Rothe, C., Hintschich, S., Palsson, L., & Monkman, A. (2003). Pressure dependent radiative quantum yields of the prompt and delayedluminescence of polyfluorene films (vol 360, pg 111, 2002). Chemical Physics Letters, 373(5-6), 646-647. https://doi.org/10.1016/s0009-2614%2803%2900634-1
• Knaapila, M., Torkkeli, M., Jokela, K., Kisko, K., Horsburgh, L., Palsson, L., …Serimaa, R. (2003). Diffraction analysis of highly ordered smectic supramolecules of conjugated rodlike polymers. Journal of Applied Crystallography, 36(3), 702-707. https://doi.org/10.1107/s0021889803003881
• Cross, G., Hackman, N., Thomas, P., Szablewski, M., Pålsson, L., & Bloor, D. (2003). Local field and aggregation dependence of the micro- and macroscopic optical non-linearity of zwitterionic molecules. Optical Materials, 21(1-3), 29-37. https://doi.org/10.1016/s0925-3467%2802%2900108-8
• Knaapila, M., Lyons, B., Kisko, K., Foreman, J., Vainio, U., Mihaylova, M., …Monkman, A. (2003). X-ray Diffraction Studies Of Multiple Orientation In Poly(9,9-bis(2-ethylhexyl)fluorene-2,7-diyl) Thin Films. Journal of Physical Chemistry B (Soft Condensed Matter and Biophysical Chemistry), 107(45), 12425-12430. https://doi.org/10.1021/jp035259t
• Rothe, C., Palsson, L., & Monkman, A. (2002). Singlet and triplet energy transfer in a benzil-doped, light emitting,solid-state conjugated polymer. Chemical Physics, 285(1), 95-101
• Palsson, L., Beavington, R., Frampton, M., Lupton, J., Magennis, S., Markham, J., …Samuel, I. (2002). Synthesis and excited state spectroscopy of tris (distyrylbenzenyl)amine-cored electroluminescent dendrimers. Macromolecules, 35(21), 7891-7901. https://doi.org/10.1021/ma011996g
• Knaapila, M., Ikkala, O., Torkkeli, M., Jokela, K., Serimaa, R., Dolbnya, I., …Monkman, A. (2002). Polarized luminescence from self-assembled, aligned, and cleaved supramolecules of highly ordered rodlike polymers. Applied Physics Letters, 81(8), 1489-1491. https://doi.org/10.1063/1.1499222
• Ikkala, O., Knaapila, M., Torkkeli, M., Palsson, L., Horsburgh, L., Jokela, K., …Monkman, A. (2002). Novel route for polarized luminance: Cleaving side groups from alignedself-organized supramolecules
• Rothe, C., Hintschich, S., Palsson, L., Monkman, A., Guentner, R., & Scherf, U. (2002). Pressure dependent radiative quantum yields of the prompt and delayedluminescence of polyfluorene films. Chemical Physics Letters, 360(1-2), 111-116
• Palsson, L., Wang, C., Russell, D., Monkman, A., Bryce, M., Rumbles, G., & Samuel, I. (2002). Photophysics of a fluorene co-polymer in solution and films. Chemical Physics, 279(2-3), 229-237. https://doi.org/10.1016/s0301-0104%2802%2900407-x
• Monkman, A., Pålsson, L., Higgins, R., Wang, C., Bryce, M., Batsanov, A., & Howard, J. (2002). Protonation and subsequent intramolecular hydrogen bonding as a method to control chain structure and tune luminescence in heteroatomic conjugated polymers. Journal of the American Chemical Society, 124(21), 6049-6055. https://doi.org/10.1021/ja012409%2B
• Varnavski, O., Samuel, I., Palsson, L., Beavington, R., Burn, P., & Goodson, T. (2002). Investigations of excitation energy transfer and intramolecular interactions in a nitrogen corded distrylbenzene dendrimer system. The Journal of Chemical Physics, 116(20), 8893-8903. https://doi.org/10.1063/1.1471241
• Bloor, D., Kagawa, Y., Szablewski, M., Ravi, M., Clark, S., Cross, G., …Rumbles, G. (2001). Matrix dependence of light emission from TCNQ adducts. Journal of materials chemistry, 11(12), 3053-3062. https://doi.org/10.1039/b104992p
• Whitelegg, S., Buckley, A., Rahn, M., Fox, A., Bradley, D., Palsson, L., …Burn, P. (2001). Time-resolved PL studies of partially conjugated MEH-PPV - Control ofexcimer emission. Synthetic Metals, 119(1-3), 575-576
• Palsson, L., Wang, C., Monkman, A., Bryce, M., Rumbles, G., & Samuel, I. (2001). Fluorene co-polymer luminescence: implications for molecular interactions. Synthetic Metals, 119(1-3), 627-628
• Bourhill, G., Palsson, L., Samuel, I., Sage, I., Oswald, I., & Duignan, J. (2001). The solid-state photoluminescent quantum yield of triboluminescentmaterials. Chemical Physics Letters, 336(3-4), 234-241
• Wang, C., Kilitziraki, M., Palsson, L., Bryce, M., Monkman, A., & Samuel, I. (2001). Polymeric alkoxy PBD [2-(4-biphenylyl)-5-phenyl-1,3,4-oxadiazole] forlight-emitting diodes. Advanced Functional Materials, 11(1), 47-50. https://doi.org/10.1002/1616-3028%28200102%2911%3A1%3C47%3A%3Aaid-adfm47%3E3.0.co%3B2-t
• Lo, S., Palsson, L., Kilitziraki, M., Burn, P., & Samuel, I. (2001). Control of polymer-electrode interactions: the effect of leaving groupon the optical properties and device characteristics of EHPPV. Journal of materials chemistry, 11(9), 2228-2231
• Peterman, E., Wenk, S., Pullerits, T., Palsson, L., van Grondelle, R., Dekker, J., …van Amerongen, H. (1998). Fluorescence and absorption spectroscopy of the weakly fluorescentchlorophyll a in cytochrome b(6)f of Synechocystis PCC6803. Biophysical Journal, 75(1), 389-398
• Palsson, L., Flemming, C., Gobets, B., van Grondelle, R., Dekker, J., & Schlodder, E. (1998). Energy transfer and charge separation in photosystem I: P700 oxidationupon selective excitation of the long-wavelength antenna chlorophyllsof Synechococcus elongatus. Biophysical Journal, 74(5), 2611-2622
• Palsson, L., Dekker, J., Schlodder, E., Monshouwer, R., & vanGrondelle, R. (1996). Polarized site-selective fluorescence spectroscopy of thelong-wavelength emitting chlorophylls in isolated Photosystem Iparticles of Synechococcus elongatus. Photosynthesis Research, 48(1-2), 239-246
• Gobets, B., Ericsson, M., Palsson, L., Shubin, V., Karapetyan, N., VanGrondelle, R., & Dekker, J. (1996). Site selected polarized fluorescence spectroscopy of photosystem Iparticles isolated from the cyanobacteria Spirulina platensis. Progress in Biophysics and Molecular Biology, 65, PE221-PE221
• Palsson, L., Gobets, B., Fleming, C., Ericsson, L., VanGrondelle, R., Schlodder, E., & Dekker, J. (1996). Long-wavelength antenna pigments in photosystem I. Progress in Biophysics and Molecular Biology, 65, PE217-PE217
• Tjus, S., Roobolboza, M., Palsson, L., & Andersson, B. (1995). RAPID ISOLATION OF PHOTOSYSTEM-I CHLOROPHYLL-BINDING PROTEINS BYANION-EXCHANGE PERFUSION CHROMATOGRAPHY. Photosynthesis Research, 45(1), 41-49
• Palsson, L., Tjus, S., Andersson, B., & Gillbro, T. (1995). ULTRAFAST ENERGY-TRANSFER DYNAMICS RESOLVED IN ISOLATED SPINACHLIGHT-HARVESTING COMPLEX-I AND THE LHC-I-730 SUBPOPULATION. BBA - Bioenergetics, 1230(1-2), 1-9
• Palsson, L., Tjus, S., Andersson, B., & Gillbro, T. (1995). ENERGY-TRANSFER IN PHOTOSYSTEM-I - TIME-RESOLVED FLUORESCENCE OF THENATIVE PHOTOSYSTEM-I COMPLEX AND ITS CORE COMPLEX. Chemical Physics, 194(2-3), 291-302
• Palsson, L., Spangfort, M., Gulbinas, V., & Gillbro, T. (1994). Ultrafast chlorophyll b-chlorophyll a excitation energy transfer in the isolated light harvesting complex, LHC II, of green plants. FEBS Letters, 339(1-2), 134-138. https://doi.org/10.1016/0014-5793%2894%2980400-1
• Palsson, L., Miller, M., & Gillbro, T. (1991). ENERGY-TRANSFER KINETICS IN THE GREEN BACTERIUM CHLOROFLEXUS-AURANTIACUS