Bioimaging Facility: Advanced Light Microscopy
The state of the art Bioimaging and Microscopy research and teaching facility in the Department of Biosciences is at the core of the Durham Centre for Bioimaging Technology and houses an extensive selection of imaging modalities
Super-Resolution Microscopes
GE Healthcare OMX V4.0
Location: Biosciences room 138
The system is an Applied Precision OMX BLAZE Super-Resolution microscope supplied by GE Healthcare. The microscope can use two light based modalities to achieve multichannel super-resolution (SRM) or sub-diffraction imaging below 200nm. The first is 3D-SIM (Structured illumination Microscopy) which provides an 8 fold improvement in volumetric resolution (xy 100nm by z 250nm) using a wide variety of common fluorophores at high frame rates allowing live cell SRM. Secondly the system is also equipped with Deltavision localisation microscopy, previously known as Monet, allowing techniques such as PALM and dSTORM which provide lateral resolution of 20-50nm, <100nm axial
3D-SIM BLAZE acquisition time: 16 3D-SIM sections (0.125nm, 2um) in 1 sec (240 images).
OMX Speed Calculator - OMX @ HMS
OMX Filter Spectra - OMX @ HMS
Lens | N.A |
---|---|
Olympus UPlan FL N 40x Oil | 1.3 |
Olympus UPlan S Apo 60x Silicone | 1.3 |
Olympus PlanApo N 60x Oil | 1.42 |
Structured Illumination Solid-state Laser Lines
405nm / 445nm / 488nm / 514nm / 568nm / 642nm
Emission filters are arranged in two filter set "drawers"
BGR Filter Set | Wavelength | CYR Filter Set | Wavelength |
---|---|---|---|
DAPI | 436 / 31nm | CFP | 478 / 35nm |
A488 / FITC | 528 / 48nm | YFP | 541 / 22nm |
A568 | 609 / 37nm | A568 / mCherry | 609 / 37nm |
Cy5 | 683 / 40nm |
Lens | N.A |
---|---|
Olympus UPlan FL N 40x Oil | 1.3 |
Olympus UPlan S Apo 60x Silicone | 1.3 |
Olympus PlanApo N 60x Oil | 1.42 |
Olympus TIRF 60x ApoN | 1.49 |
Olympus TIRF 100x UApoN | 1.49 |
Conventional Wide-field Light Source | Excitation Range |
---|---|
DAPI | 381 - 410 nm |
CFP | 426 - 450 nm |
FITC /GFP | 461 - 493 nm |
YFP | 505 - 520 nm |
A568 / mCherry | 562 - 581 nm |
Cy 5 | 638 - 653 nm |
Emission filters are arranged in teo filter set "drawers"
BGR Filter Set | Wavelength | CYR Filter Set | Wavelength |
---|---|---|---|
DAPI | 436 / 31nm | CFP | 478 / 35nm |
A488 / FITC | 528 / 48nm | YFP | 541 / 22nm |
A568 | 609 / 37nm | A568 / mCherry | 609 / 37nm |
Cy5 | 683 / 40nm |
The OMX includes a novel Ring TIRF module - this rotates the laser beam rapidly at the back focal plane of the objective lens creating a highly uniform TIRF illumination.
TIRF mode is utilised for DeltaVision Localization Microscopy - capable of resolution ~20-30nm
Lens | N.A |
---|---|
Olympus TIRF 60x ApoN | 1.49 |
Olympus TIRF 100x UApoN | 1.49 |
Conventional Wide-field Light Source | Excitation Range |
---|---|
DAPI | 381 - 410 nm |
CFP | 426 - 450 nm |
FITC /GFP | 461 - 493 nm |
YFP | 505 - 520 nm |
A568 / mCherry | 562 - 581 nm |
Cy 5 | 638 - 653 nm |
Emission filters are arranged in two filter set "drawers"
BGR Filter Set | Wavelength | CYR Filter Set | Wavelength |
---|---|---|---|
DAPI | 436 / 31nm | CFP | 478 / 35nm |
A488 / FITC | 528 / 48nm | YFP | 541 / 22nm |
A568 | 609 / 37nm | A568 / mCherry | 609 / 37nm |
Cy5 | 683 / 40nm |