Location: Biosciences room 138

The system is an Applied Precision OMX BLAZE Super-Resolution microscope supplied by GE Healthcare. The microscope can use two light based modalities to achieve multichannel super-resolution (SRM) or sub-diffraction imaging below 200nm. The first is 3D-SIM (Structured illumination Microscopy) which provides an 8 fold improvement in volumetric resolution (xy 100nm by z 250nm) using a wide variety of common fluorophores at high frame rates allowing live cell SRM. Secondly the system is also equipped with Deltavision localisation microscopy, previously known as Monet, allowing techniques such as PALM and dSTORM which provide lateral resolution of 20-50nm, <100nm axial

3D-SIM BLAZE acquisition time: 16 3D-SIM sections (0.125nm, 2um) in 1 sec (240 images).

OMX Speed Calculator - OMX @ HMS

OMX Filter Spectra - OMX @ HMS

Structured Illumination Solid-state Laser Lines

405nm / 445nm / 488nm / 514nm / 568nm / 642nm

Emission filters are arranged in two filter set "drawers"

Emission filters are arranged in teo filter set "drawers"

The OMX includes a novel Ring TIRF module - this rotates the laser beam rapidly at the back focal plane of the objective lens creating a highly uniform TIRF illumination.

TIRF mode is utilised for DeltaVision Localization Microscopy - capable of resolution ~20-30nm

Emission filters are arranged in two filter set "drawers"