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Bioimaging Facility: Advanced Light Microscopy

The state of the art Bioimaging and Microscopy research and teaching facility in the Department of Biosciences is at the core of the Durham Centre for Bioimaging Technology and houses an extensive selection of imaging modalities

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Zeiss Axiovert 200M Apotome

Location: Biosciences Room 138

The Apotome is a widefield fluorescence microscope which allows you to create optical sections of your samples free of scattered light. With optical sectioning structured illumination (OS-SIM) only the focal plane appears in your final image. The Apotome projects a grid structure onto the focal plane of your specimen, then moves it into three positions using a scanning mechanism acquiring an image at each position. The system processes the three images into one optical section with improved contrast and increased resolution. The process occurs automatically making this as easy to use as an standard widefield fluorescence microscope.

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Zeiss 10x EC Plan NEOFLUAR PH1 0.3
Zeiss 20x Plan-APOCHROMAT PH2 0.8
Zeiss 40x LD Plan NEOFLUAR PH2 Korr 0.6
Zeiss 63x LD Plan NEOFLUAR PH2 Korr 0.75
Zeiss 63x Plan-APOCHROMAT Oil PH3 1.4
Zeiss 100x Plan-APOCHROMAT Oil PH3 1.4


Filter CubeExcitationEmission
DAPI 335 - 383 nm 420 LP
Alexa Fluor 488 450 - 490 nm 515 LP
HE YFP 482 - 512 nm 520 - 550 
Alexa Fluor 546 540 - 552 nm 590 LP